Transmission Electron Microscopy Methods for Understanding the Brain by Elisabeth J. Van Bockstaele

Author:Elisabeth J. Van Bockstaele
Language: eng
Format: epub, pdf
Publisher: Springer New York, New York, NY

3.2 Materials

Reagents

Phosphate-buffered saline (Difco FA buffer Becton, Dickinson, MA).

8 % glutaraldehyde (Electron Microscopy Sciences EMS, Hatfield, PA).

Paraformaldehyde in Prills (Sigma Aldrich, St. Louis, MO).

Bovine serum albumin (BSA-e Aurion EMS).

Rabbit antibody to Abeta 1-42 AP5078P (Millipore, Temecula, CA).

Goat anti-rabbit antibody conjugated to 10 nm colloidal gold (Aurion EMS).

10 % hydrogen peroxide, molecular biology-grade water (Sigma-Aldrich) (see Section 3.4, Note 1).

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